Material: PEEK

Colour: Natural/Beige

Maximum Temperature: 260°C/500°F

Flammability: UL94 V-0

HPLC PEEK Flangeless Nut, Flat bottom (5/16-24 UNF for 3/16" OD Tubing) conform to the following Standards/Norms:

HPLC flangeless PEEK fittings are designed for connecting 3/16" capillary tubing in High-Performance Liquid Chromatography (HPLC) systems. These fittings provide a reliable and leak-free connection between the tubing and various components of the HPLC system, such as detectors, columns, and injection valves.

Thread size: #5/16-24 UNF

Thread length: 0.39” (10mm)

Material: PEEK

Features:  Finger-tight fitting / Flat bottom.

Finger-tight fitting:

Versatility: Compatible with a wide range of tubing sizes and materials, offering flexibility in experimental setups and accommodating different application requirements.

Minimized Risk of Damage: Controlled tightening reduces the risk of over-tightening, which can damage fittings or tubing, thereby extending the lifespan of equipment and reducing maintenance costs.

Flat bottom:

Reduced Dead Volume: The flat bottom design minimises dead volume within the fitting, optimising chromatographic performance by improving sample resolution and peak shape.

Uniform Compression: Ensures even distribution of force during tightening, enhancing sealing efficiency and reliability.

Manufactured in PEEK:

Chemical Resistance: PEEK is highly resistant to a wide range of chemicals, solvents, and acids commonly used in HPLC applications. This ensures compatibility with various mobile phases without degradation or leaching, maintaining sample integrity.

Inertness: PEEK is inert and non-reactive, minimising the risk of introducing contaminants or altering sample composition during chromatographic analysis. This property is crucial for ensuring accurate and reliable analytical results.

Temperature Stability: PEEK exhibits excellent thermal stability, maintaining its dimensional integrity and performance characteristics across a broad temperature range typically encountered in HPLC systems. It can withstand both high and low temperatures without compromising performance.

Mechanical Strength: PEEK possesses high mechanical strength and stiffness, providing robustness and durability to the flangeless nut. This strength ensures reliable sealing and prevents leaks under operating pressures in HPLC systems.

Dimensional Stability: PEEK maintains dimensional stability even under fluctuating temperature and pressure conditions, ensuring consistent performance and a secure fit over time. This property contributes to the reliability and longevity of the chromatographic system.

Biocompatibility: PEEK is biocompatible and widely used in medical and pharmaceutical applications. In HPLC, this property ensures that the material does not interact with biological samples or pharmaceutical compounds, maintaining sample purity and safety.

HPLC PEEK Flangeless Nut, Flat Bottom applications:

Column Connections: These fittings are used to connect chromatography columns (e.g., analytical, preparative, guard columns) to the HPLC system. They ensure a secure and leak-free connection between the column and the tubing, crucial for maintaining chromatographic performance and integrity.

Detector Connections: HPLC systems often require precise connections between detectors (such as UV-Vis detectors, fluorescence detectors, or mass spectrometers) and the chromatographic flow path. PEEK flangeless nut, flat bottom fittings provide reliable sealing to prevent leaks and ensure accurate detection of analytes.

Sample Injection Systems: In automated or manual sample injection systems (such as autosamplers or manual injectors), these fittings connect injection valves or sample loops to the chromatographic flow path. They facilitate precise and reproducible sample introduction without introducing dead volume or leaks.

Flow Splitters and Dividers: For applications requiring the splitting or diverting of flow between multiple detectors or columns, PEEK flangeless nut, flat bottom fittings ensure efficient distribution of the chromatographic flow while maintaining system integrity and performance.

Buffer and Mobile Phase Delivery: These fittings are used to connect tubing carrying mobile phases or buffer solutions to the chromatographic system. They provide a secure and reliable connection that withstands the pressure and flow requirements of HPLC operation.

Fraction Collection Systems: In fraction collection systems, PEEK flangeless nut, flat bottom fittings connect tubing from the column outlet to fraction collectors. They ensure precise and leak-free collection of separated analytes or fractions, crucial for downstream analysis or purification processes.

Valve and Pump Connections: These fittings connect tubing to valves (such as switching valves or injection valves) and pumps within the HPLC system. They facilitate smooth operation and accurate flow control, minimising system downtime and ensuring consistent performance.

Analytical and Preparative HPLC: Whether in analytical laboratories performing routine analyses or in preparative chromatography for isolating compounds on a larger scale, PEEK flangeless nut, flat bottom fittings play a critical role in maintaining the integrity and efficiency of chromatographic separations.

Environmental and Food Testing: In environmental monitoring and food safety laboratories, these fittings are used in HPLC systems for analysing contaminants, pesticides, and other compounds. They ensure reliable and accurate detection while minimizing sample contamination risks.

Biomedical Research: In biomedical research, including proteomics, metabolomics, and pharmaceutical analysis, these fittings are employed for analysing biomolecules and pharmaceutical compounds with high sensitivity and accuracy, crucial for advancing scientific understanding and drug development.

The PEEK data sheet is available as a PDF to download below.

Return components within 90 days for a full refund. Please see returns terms and conditions for further information.